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Journal: PLOS Biology
Article Title: Cdc42 interacts with chaperone Ydj1 to enhance its stability and partitioning during asymmetric cell division and aging in yeast
doi: 10.1371/journal.pbio.3003306
Figure Lengend Snippet: A. Cdc42-mCherry SW levels in each pair of young and old mothers and their daughters during M-cytokinesis ( n = 37 pairs, each group). **** p < 0.0001 from paired t-tests. Refer to legend. B. Cdc42-mCherry SW levels in mother and daughter pairs at the final division preceding death of both cells (see Cell 2 in ). n = 7 (out of 37 lineages); ns, p ≥ 0.05, paired t test. C. Schematic diagrams of GFP-Cdc42 and Cdc42-ritC-GFP with the Rit amphipathic helix (in blue). Levels of GFP-Cdc42 and Cdc42-ritC-GFP were compared by immunoblotting extracts from cells (expressing each allele as the sole genomic copy) grown at 25 °C to early log phase, using a monoclonal anti-GFP antibody and α-tubulin as a loading control. The mean GFP fluorescence in whole cells (mother and bud combined) of these strains, grown at 25 °C, is shown in a Tukey plot ( n = 72 per strain); ns, p ≥ 0.05, unpaired t t est. D. Localization of Cdc42-ritC-GFP at 25 °C, and quantification in mother and bud compartments during M-cytokinesis. Twenty representative pairs are shown. ns, p ≥ 0.05, paired t test. Scale bar: 3 µm. See and . The data underlying the graphs can be found in .
Article Snippet: Proteins were separated by SDS-PAGE on 12.5% polyacrylamide gels, and western blots were visualized using the LI-COR Odyssey system (LI-COR Biosciences, Lincoln, Nebraska) with the following antibodies: mouse monoclonal anti-Cdc42 antibody (clone 28-10) (EMD Millipore, MABN2485), rabbit polyclonal anti-RFP antibodies (Rockland, 600-401-379),
Techniques: Western Blot, Expressing, Control, Fluorescence